Biocytin molecular weight
WebSep 30, 2015 · Notably, biocytin, a low molecular weight conjugate of biotin and lysine, has found multiple uses as an antero- and retrograde neuroanatomical tracer, and in labeling individual neurons using intracellular, whole-cell patch or juxtacellular techniques (Kobbert et al., 2000, Thomson and Armstrong, 2011). WebAF 594 biocytin is a cell-impermeant fixable red fluorescent polar tracer that can be used to examine plasma membrane permeability, pinocytosis, transport through gap junctions, and cell-cell and cell-liposome fusion. ... Molecular weight: 1279.66 Molecular formula: ...
Biocytin molecular weight
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WebMolecular Weight: 244.31. Isoelectric pH = 3.5. Biotin is: water-soluble; soluble in dilute alkali and hot water; relatively stable at acidic and neutral pH range; Biocytin: Freely … WebBiocytin has been used as an intracellular labeling reagent for neurons. 2-12 Advantages of using biocytin include its high solubility in aqueous solutions and small molecular weight, which facilitate its injection using micropipettes. Lucifer yellow, a fluorescent dye used for labeling neurons, can clog microelectrodes more readily than ...
WebDefinitions of molecular mass, molecular weight, molar mass and molar weight: Molecular mass (molecular weight) is the mass of one molecule of a substance and is … WebThe invention generally relates to compositions and methods for transporting substances across mucosal barriers. The invention also relates to methods of making and using such substances.
WebMolecular weight. 608.63. Biocytin maleimide readily reacts with thiol moieties of biopolymers to form thioether conjugate that is quite stable. This biocytin maleimide requires mild conjugation conditions. For example, pH of 5.5-8.5 is usually optimal for modifying cysteine residues, and air exposure of reaction solution should be minimized ... WebBiocytin is a versatile marker used in anterograde, retrograde and intracellular neuroanatomical investigations and in biotinidase assays. ... The following data is based …
WebSep 18, 1989 · Biocytin, a naturally occurring low molecular weight analog of biotin, was evaluated as a neuroanatomical tract-tracing marker in the adult rat brain. Since it retains high-affinity binding to avidin, biocytin can be labelled with avidinylated visualization reagents. Iontophoretic or pressure injections resulted in filling of cell bodies and ...
WebMar 7, 2011 · We sought to determine the involvement of phosphatidyl inositol 3-kinase (PI3K) and AMP-activated protein kinase (AMPK) in the estrogenic antagonism of the cannabinoid regulation of energy homeostasis. Food intake and body weight were evaluated in ovariectomized female guinea pigs treated s.c. with estradiol benzoate (EB) … solar power for gardenWebBiocytin is a combination of biotin and lysine, which is very small and soluble. In neuroanatomy research, it is widely used as a neuroanatomy tracer. N6- [5- [ (3aS,4S,6aR)-Hexahydro-2-oxo-1H-thieno [3,4-d]imidazol-4-yl]-1-oxopentyl]-L-lysine. Biocytin is absorbed by neurons and transported rapidly in both forward and retrograde directions. sly cooper 2 wikiWebMar 9, 2015 · Biocytin is a versatile marker used for neuroanatomical studies and biotinidase assays. Biocytin is water soluble and can be easily detected by avidin and … sly cooper 2 safe locationsWeb576-19-2. Molecular weight. 372.48. Biocytin is invaluable as a rapidly transporting, sensitive neuronal tracer requiring little permeabilizing agents. It is an especially versatile … sly cooper 3 cheat codes ps3WebAs well as a molecular force probe for atomic force microscopy studies, novel materials such as 3D crystalline lattices have also been created. Streptavidin has a mildly acidic … sly cooper 3 emulatorWebThe likelihood that primary amines are present increases as molecular weight increases. ... Biocytin is invaluable as a rapidly transporting, sensitive neuronal tracer requiring little permeabilizing agents. It is an especially versatile marker for neuroanatomical investigations. Biocytin may be injected into brain by iontophoresis or by ... sly cooper 3 charactersWebBiocytin is a conjugate of D-biotin and L-lysine, where the carboxylate of D-biotin is coupled with the ϵ-amine of L-lysine via a secondary amide bond. Biocytin is a classical … solar power for house off the grid