Dld1 puromycin
WebDec 7, 2016 · DLD1 cells were selected using 2 μg/ml puromycin to establish stable cell lines. Western blot analysis. WebThermo Fisher dld1 puromycin Dld1 Puromycin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS …
Dld1 puromycin
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WebDec 18, 2024 · DLD1, HT-29, and LoVo cells were cultured in RPMI 1640 medium (Gibco, USA), and FHC and SW480 cells were cultured in DMEM/high-glucose medium (Gibco, USA). ... The CRC cells with stable circIL4R knockdown were selected with puromycin and verified by qRT–PCR. The sequences of the siRNA, shRNA and plasmids are described … Web1 day ago · Either DLD1 cells or U2OS cells were transduced with lentivirus to stably integrate a puromycin-resistant construct encoding 3×Flag-tagged dCas9 and a hygromycin-resistant construct encoding ...
WebPuromycin; 2μg/ml; 796: DLD-1: pUHD15-1neo; pUHD10-3hygro; Geneticin (G418) Hygromycin; Tetracycline; 200μg/ml; 200μg/ml; 1μg/ml; 890: DLD-1: pcDNA3.1; … WebJan 2, 2024 · DLD1 BRCA2 −/− is a colorectal adenocarcinoma cell line with homozygous deletion of exon 11 of the BRCA2 gene , engineered from BRCA2 wild-type DLD1 cells. The use of isogenic cell pairs allowed us to exclude many other unknown factors that could contribute to the differential sensitivity in nonisogenic systems.
WebTrans IT®-2024 is the only reagent on the market that fits those criteria. In addition, Trans IT®-2024 also transfects colon cancer cell lines HCT-116 and DLD1 with much higher efficiencies. Overall, the pace and quality of our research have improved since we started using Trans IT®-2024. John A. Burns. WebPuromycin is an aminonucleoside antibiotic produced by the bacterium Streptomyces alboniger. During puromycin selection, this antibiotic inhibits cellular protein synthesis by …
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WebCOLO 320DM. CCL-220 ™. COLO 320DM is a rounded and refractile cell line that was isolated from the colon of a White, 55-year-old, female patient with colorectal adenocarcinoma cancer. This cell line was deposited by GE Moore and can be used in cancer research. Product category. map of north yorkshire towns and villagesWebDeleted in Liver Cancer 1 also known as DLC1 and StAR-related lipid transfer protein 12 (STARD12) is a protein which in humans is encoded by the DLC1 gene.. This gene is … map of north yorkshire moors national parkWebMay 22, 2024 · DLD1 BRCA2 (−/−) cells were individually cotransfected with the BRCA2 constructs and transposase expression vector. After puromycin selection of stable transfectants, equal numbers of DLD1 ... map of norton fitzwarrenWebA PD-L1 test uses a sample of cancerous tumor tissue to measure how much of a protein called PD-L1 is found on the cancer cells. If you have certain types of cancer, PD-L1 … map of north yorkshire coastal townsWebStable cell lines were selected in RPMI 1640 medium containing 2 μg/mL puromycin (Sigma-Aldrich Co., St Louis, MO, USA) and confirmed by Western blot analysis. ... So SW480 and DLD1 cells were chosen to adopt overexpression of STC2, whereas HCT116 and RKO cells were chosen to adopt inhibition of STC2. Then we conducted the … map of north yorkshire districtsWebOct 22, 2024 · After 48 h, cells were selected in puromycin (10 ug/mL) for 24 h and maintained in 1 ug/mL puromycin until single clones were isolated. Screening for CerS protein expression knock-out was conducted by Western blot analysis using the appropriate CerS antibodies. ... Conditioned media from M1 macrophages/DLD1 cells co-culture as … map of northwood universityWebSep 1, 2024 · To further determine the apoptosis-inducing effect of SPP on DLD-1 cells, Annexin V-FITC/PI was used to stain DLD-1 cells, and it was found that the number of … kropp recyclinghof