Dnase i qiagen
WebFrozen testis tissues were homogenized, and total RNA extracted using the miRNeasy Micro Kit (Qiagen, Germany), according to the manufacturer’s protocol. ... 1 μL of each primer and 7.0 μL of DNase/RNase-free water for the gene expression profile. β-actin was used as the reference gene and DGCR8 also as the target gene, ... Web1.2 ug of DNA was digested with DNase I for 12 minutes. The digested DNA was tagged using that BioPrime Array CGH Genomic Labeling Element with Amersham cUTP-CY dyes upon GE Healthcare (Reference=CY3 and Tumor=CY5). The labeled DNA were purified using Vivaspin 500 columns : Channel 2: Sourced name: Promega XX Control DNA:
Dnase i qiagen
Did you know?
Web1. Add 0.1 volume (5.6 µL) of 10X DNase I buffer and 1 µL of DNase I (2 units) to the RNA. Mix gently by flicking tube (DO NOT VORTEX) and incubate in a 37oC water bath for 20 minutes. 2. Add 0.1 volume of DNAse Inactivation Reagent (6.17 µL) to the RNA. (Make … WebOur lab uses DNase I from Ambion--it comes also as 1500 units. We basically just have to add a special 10x buffer, then the RNA, then we do a sequential 0.5 ul DNase I incubation at 37 degrees for 30 minutes followed by 0.5 ul more of DNAse I at 37 degrees for 30 …
WebTotal RNA was isolated using Qiagen RNeasy kit following manufacturer's protocol and including on-column DNase-digestion. RNA where quantifiably with UV-spec Nanodrop and than proficient on Agilent Bioanalyzer. Label: Cy5: Tag protocol: WebFor simulcast purification of DNA and RNA from cellular additionally tissues, inclusive small samples, in spin-column and 96-well format
WebApr 14, 2024 · QIAGEN 1027284 AllStars Neg. siRNA AF 488 (5 nmol) QIAGEN 1027272 miScript Negative Control Inhititor(20) QIAGEN 9233431 Belt, tooth, handler z, BR8000 QIAGEN 9019232 Opto - Y, PyroQ24 QIAGEN 1064143 DNase Booster (2/2), KG QIAGEN 1061086 TB Quantification Standards WebDNase Reagents as Required: DNaseI (New England Biolabs) 31243 (or BDP-approved equivalent) EDTA (0.5M, pH 8.0) 10098 (or BDP-approved equivalent) DNA/RNA Extraction Reagents as Needed: QIAGEN DNA Mini Kit 30442 QIAGEN Blood Mini Kit 30444 …
WebDNAse digestion (Qiagen Rnase-Free Dnase Set, Cat #79254) 1. Start with <87.5 µl (no more than 100ug total RNA) of crude RNA preparation. 2. Add 10 µl of Buffer RDD and 2.5 µl of DNase I stock solution. 3. Make the volume up to 100 µl with RNase-free water. 4. …
WebTotal RNA was extracted from the same tissues using the RNeasy Plant Mini kit (Qiagen #74904) with RNase-free DNase set (Qiagen #79254). Reverse transcription (RT) was performed on 1 μg of DNase-treated total RNA using the iScript cDNA synthesis kit (Bio … mercy health barberton ohioWebProducts. Blueprint your experiment; Discovery & Translative Research. DNA & RNA Purification. DNA. Cell-Free DNA; DNA Clean Up; Genomic DNA; Microbial DNA; Plasmid DNA mercy health bakersfield caWebThe solutions were then treated with RNase-Free DNase I (Promega) by addition of 10x Reaction Buffer (400 mM Tris-HCl, pH 8.0 at 25°C, ... (QIAGEN) or the NucleoSpin Gel and PCR Cleanup Kit (Takara) according to the manufacturer’s protocol. In-Fusion cloning … mercyhealth barb bortnerWebThe On-Column DNase I Digestion Set offers a simple and convenient method for eliminating genomic DNA from total RNA preparations. This Set is intended for use with the GenElute ™ Mammalian Total RNA Miniprep Kit, the GenElute Bacterial Total RNA … how old is nicole nalepaWebThe solutions were then treated with RNase-Free DNase I (Promega) by addition of 10x Reaction Buffer (400 mM Tris-HCl, pH 8.0 at 25°C, ... (QIAGEN) or the NucleoSpin Gel and PCR Cleanup Kit (Takara) according to the manufacturer’s protocol. In … mercy health bariatric clinicWebThe RNase-Free DNase Set provides efficient on-column digestion of DNA during RNA purification from cells and tissues using RNeasy Kits and the QIAamp RNA Blood Mini Kit. The DNase is efficiently removed in subsequent wash steps. For DNase treatment when … how old is nicole oulsonWebApr 8, 2024 · Oxaliplatin-sensitivity was detected via using the succinate dehydrogenase inhibition (SDI) assay as developed. Briefly, the tumor with removed necrotic tissues from colon cancer patients following oxaliplatin-based chemotheraphy was cultured in the … how old is nicole laeno 2022