Web胞内染色步骤. 1. 根据上述说明固定组织样本. 2. 加入 100 µL 洗涤剂为基础的穿透剂，在室温下避光孵育 15 min. 3. 用 2 mL PBS（含 0.1% triton 或其它穿透洗涤剂）洗涤细胞，以 … WebApr 17, 2009 · Generically, people expand this to Mean Fluorescence Intensity, but ironically, you’d rarely use the actual Mean of the population. Basically what the MFI is …
流式细胞术概述 Cell Signaling Technology
http://www.ichacha.net/flow%20cytometry.html Web胞内染色步骤. 1. 根据上述说明固定组织样本. 2. 加入 100 µL 洗涤剂为基础的穿透剂，在室温下避光孵育 15 min. 3. 用 2 mL PBS（含 0.1% triton 或其它穿透洗涤剂）洗涤细胞，以 300 g (2,000 rpm) 的速度离心 5 min，弃去上清液，重悬剩余物中的沉淀. 4. 按照直接和间接实验 ... crystal crown judged trail ride
Flow cytometry intracellular staining protocol Abcam
WebKey features and details. Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply. Alexa Fluor® 488 Rabbit monoclonal [SP216] to Sialoadhesin/CD169. Suitable for: Flow Cyt (Intra), IHC-P. Reacts with: Human. WebIntracellular staining procedure. Add 100 µL detergent-based permeabilizing agent and incubate in the dark at room temperature for 15 min. Wash the cells with 2 mL of PBS (containing 0.1% triton or other permeabilizing detergent), centrifuge at 300 x g (2,000 … WebOct 30, 2024 · 其实在选择正确抗体的道路上，存在很多“坑”，只要成功避开，就可以选到适合您实验的抗体。. 今天就来和大家分享一下“如何选择正确的抗体”。. 1、蛋白的名称和 … dwarf nation name generator