Phospholipidosis detection reagent

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August undefined, 2024

WebSep 3, 2012 · Drug-induced phospholipidosis (PLD) is a lysosomal storage disorder characterized by the accumulation of phospholipids within the lysosome. ... After 48 h, the medium was replaced with fresh medium that included the test substances and HCS LipidTOX Green phospholipidosis detection reagent (Invitrogen, San Diego, US) at their …

Drug-Induced Phospholipidosis: Best Practices and Screening

WebMar 1, 2024 · LipidTOX Green Phospholipidosis Detection Reagent (LipidTOX, Invitrogen, Carlsbad, CA, USA) was applied to confirm induction of phospholipidosis by selected drugs. According to the manufacturer's protocol, LipidTOX was diluted in full media (1:500) and then filtered with a syringe filter (2 μm in pores size). ... WebJan 1, 2014 · Phospholipidosis (PLD) is characterized by excessive intracellular accumulation of phospholipids, with a distinct morphological appearance of lamellar bodies visible under an electron microscope (EM). 1 Drugs, including many antibiotics, antidepressants, and antihistamines, induce PLD in various tissues such as lung, 2 heart, … dvr with digital antenna

HCS LipidTOX Phospholipidosis and Steatosis …

WebHCS LipidTOX™ Phospholipidosis and Steatosis Detection Kit offers a complete set of reagents for performing image-based high-content screening (HCS) assays to characterize phospolipidosis and steatosis, toxic side effects on lipid metabolism that can be triggered by drugs or other compounds. WebInitial experiments focused on the response of human mesothelioma cells to agents known to cause drug-induced phospholipidosis. When H-meso cells were treated with various concentrations of either chloroquine or verapamil a dose dependent increase in Lyso-ID Red signal is observed (Figure 5). WebThe HCS LipidTOX Red phospholipidosis detection reagent was developed to characterize the potentially toxic side effects of compounds on lipid metabolism in mammalian cell … crystal castles pinout

HCS LipidTOX™ Red Neutral Lipid Stain, for cellular imaging

HCS LipidTOX™ Red Phospholipidosis Detection Reagent …

WebThe HCS LipidTOX™ Red phospholipidosis detection reagent was developed to characterize the potentially toxic side effects of compounds on lipid metabolism in mammalian cell … WebPhospholipidosis is an excessive intracellular accumulation of phospholipids and drug, which is normally reversible after discontinuation of drug treatment. Currently, it is … dvr with hard drive for tvWebDec 7, 2024 · Annexin V-FITC/PI apoptosis detection kit was acquired from BD (Franklin Lakes, NJ, USA). Then, Hoechst 33342, eBioscience™ JC-1 Mitochondrial Membrane Potential Dye, Cell ROX Deep Red, and HCS LipidTOX™ Green Phospholipidosis Detection Reagent were obtained from Thermo Fisher Scientific (Waltham, MA, USA). dvr with hdmi input and output

"WebNov 9, 2024 · Phospholipidosis (PLD), the intracellular accumulation of phospholipids, is an adaptive response to toxic stimuli and serves as an important parameter in the biological … " - Phospholipidosis detection reagent

Phospholipidosis detection reagent

Invitrogen HCS LipidTOX Red Neutral Lipid Stain, for cellular imaging …

WebJan 27, 2024 · Briefly, cells were incubated in complete bronchial epithelial growth medium supplemented with 1 x phospholipidosis detection reagent for 48 hr. Cells were subsequently fixed in 4% PFA and stained with 1 X neutral lipid detected reagent for 30 min and counterstained with 1 μg mL-1 Hoechst 33258. Staining intensity was normalized to … WebThis probe is compatible with the HCS LipidTOX phospholipidosis detection reagents. HCS LipidTOX neutral lipid stains can also be used to monitor the formation and differentiation of adipocytes, a process called adipogenesis. Cell Analysis, Cellular Imaging, High-Content Screening (HCS) Order Info Shipping Condition: Room temperature Specifications

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WebThe HCS LipidTOX Red phospholipidosis detection reagent was developed to characterize the potentially toxic side effects of compounds on lipid metabolism in mammalian cell lines. This reagent is easily detected by fluorescence microscopy or an HCS reader equipped with filters that can detect excitation/emission maxima approx. 595/615nm ... WebInvitrogen’s HCS LipidTOX™ Phospholipidosis and Steatosis Detection Kits offer the reagents for performing image-based high-content screening (HCS) assays to …

WebThe HCS LipidTOX™ Red phospholipidosis detection reagent was developed to characterize the potentially toxic side effects of compounds on lipid metabolism in mammalian cell lines. This reagent is easily detected by fluorescence microscopy or an HCS reader equipped with filters that can detect excitation/emission maxima ∼595/615 nm. WebFeb 27, 2024 · The LipidTOX Green Phospholipidosis Detection Reagent is dedicated to phospholipidosis detection, which is often triggered by cationic amphiphilic drugs. Within 24 h of incubation with CQ, the increase in fluorescence intensity of LipidTOX for phospholipidosis detection was significant.

WebHCS LipidTOX™ Phospholipidosis Detection Reagents 3 Preparing the Labeling Solution and Staining the Cells 1.1 Dilute the 1000X LipidTOX™ phospholipidosis detection … WebNov 9, 2024 · Phospholipidosis (PLD), the intracellular accumulation of phospholipids, is an adaptive response to toxic stimuli and serves as an important parameter in the biological assessment of compounds. Cationic amphiphilic drugs are the main inducers of PLD and may impair the function of alveolar macrophages.

WebJan 1, 2014 · Phospholipidosis (PLD) is characterized by excessive intracellular accumulation of phospholipids, with a distinct morphological appearance of lamellar …

WebJun 6, 2024 · The phospholipid storage was accompanied by an expansion of the endolysosomal compartment in cultured TEC as evidenced by the increased intensity of LysoTracker Red staining and the enhanced... crystal castles remixed rewiredWebH34157 HCS LipidTOX™ Phospholipidosis and Steatosis Detection Kit *for high content screening* *for cellular imaging* *2-plate size*. . . . . 1 kit ... H34350 HCS LipidTOX™ Green phospholipidosis detection reagent *1000X aqueous solution* *for cellular imaging* 10-plate size* . . . . . . . . . each dvr without internal storageWebSeveral methods for the detection of phospholipids have been reported (29-37). They are summarized in Table 1. In-vitro screening assays using the fluorescently tagged lipid NBD-PE, the fluorescent probe Nile red as well as the phosphatidylcholine-conjugated dye NBD-PC were previously used to determine intracellular phospholipids (29, 30, 34). dvr with hard drivesWebThermo Fisherhcs lipidtox green phospholipidosis detection reagent Hcs Lipidtox Green Phospholipidosis Detection Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more crystal castles perthWebOct 30, 2024 · In this chapter, we describe fluorescent reagents and the protocols for the measurement of various parameters such as mitochondrial membrane potential, plasma … dvr without cableWebJul 16, 2015 · For phospholipid staining, the cells were treated with 400 μM palmitate containing 1X LipidTOX Red phospholipidosis detection reagent. For Immunostaining with an anti-calnexin antibody, the cells were fixed in 4 % paraformaldehyde in PBS. The fixed cells were rinsed with PBS and incubated in blocking solution (5 % goat serum, and 0.001 … dvr without cable boxWebInvitrogen™ HCS LipidTOX™ Green Phospholipidosis Detection Reagent (1000X), for cellular imaging. Easily detected by fluorescence microscopy. Manufacturer: Invitrogen™ … crystal castles robert smith