Tth ssb
WebOct 1, 2002 · The expression was induced by 0.5 mM IPTG, but it was accompanied by strong inhibition of culture growth.The best induction of thermostable SSB-like proteins … WebOct 1, 2002 · Single-stranded DNA-binding proteins (SSBs) play essential roles in DNA replication, recombination, and repair in bacteria, archaea, and eukarya.We report here the identification, expression, and purification of the SSB-like proteins of the thermophilic bacteria Thermus thermophilus and T. aquaticus.The nucleotide (nt) sequence revealed …
Tth ssb
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WebIncluding ET SSB in TtAgo reactions can improve cleavage of higher GC content substrates In some cases the addition of ET SSB ( NEB #M2401 ) can aid in the cleavage of higher GC content dsDNA substrates.1 ET SSB must be titrated to find an optimal concentration for the application, and is only found to be beneficial when guides are fully complementary to the …
WebJan 1, 2012 · We describe a procedure to detect protein binding to SSB by polyacrylamide gel electrophoresis under non-denaturing conditions. As an example, we show the … WebET SSB can be used in applications that require extremely high temperature conditions, such as nucleic acid amplification and sequencing. Figure 1: Improved multiplex PCR by ET …
WebTtAgo is a prokaryotic argonaute from the Gram-negative, thermophilic bacterium Thermus thermophilus which functions as a DNA-guided endonuclease when provided with a 16–18 nucleotide long 5′-phosphorylated single-stranded DNA oligonucleotide guide. The RNase H-like active site of TtAgo requires divalent Mg 2+ (or Mn 2+) (See product usage note #1) … WebDec 25, 2013 · NaCl, followed by a buffer wash to remove unbound Tth SSB proteins. The buffer containing the indicated Tth SSB and NaCl concentration was then. injected into …
WebApr 5, 2008 · The thermophilic bacterium Thermoanaerobacter tengcongensis has two single-stranded DNA-binding (SSB) proteins, designated TteSSB2 and TteSSB3. In a SSB complementation assay in Escherichia coli, only TteSSB3 took over the in vivo function of EcoSSB. We have cloned the ssb genes obtained by PCR and have developed E. coli …
WebSSB is known to bind ssDNA, protecting it from cleavage by endonucleases. 4 Furthermore, SSB has been found to reduce secondary structure of ssDNA, improve PCR specificity, … dick smith ge6810WebDec 25, 2013 · INTRODUCTION. Single-stranded (ss) DNA binding (SSB) proteins are ubiquitous and found in bacterial, archaeal and eukaryotic cells, mitochondria, phages and viruses ().SSB proteins bind specifically to ssDNA with high affinity in a sequence-independent manner, protect ssDNA from nucleolytic digestion and prevent intrastrand … citrus magic spot and stain removerWebExcellgen Tth-SSB [EG-171] - Return to List . Next citrus magic solid odor absorberWebSingle-Stranded DNA Binding Protein (SSB) 以高亲和力协同结合单链 DNA，其与双链 DNA 的结合度不高。与单链 DNA 结合后，SSB 使螺旋二聚体变得不稳定，因此 DNA 聚合酶可 … dick smith ge6840WebThis “step up” feature of the SSB reflects the objective of the Savings Bond programme, which is to facilitate long-term savings and investment. Coupon rates for each issuance of Savings Bonds are determined such that the average annual compounded return over an investor’s investment period (e.g. 5 years) is linked to the yield of an SGS ... dick smith ge6987WebssDNA Binding Proteins. Product Listing Product Overview. NEB offers highly thermostable ssDNA binding proteins that are ideal for nucleic acid amplification and sequencing (Tth … citrus magic spray air freshenerWebAug 7, 2014 · We therefore investigated whether a SSB protein could modify the slippage properties of Pab pols. Thermus thermophilus (Tth) SSB stimulates DNA synthesis of Tth DNA polymerase and the heterologous DNA polymerase from the Archaea P. furiosus (Perales et al., 2003). citrus magic tropical lemon air freshener